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Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The dual monochromator optics allow. 2 x 10-14. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax L: 36. Skatron Skanwasher 300 Washer. Recommended Optics Wavelength (nm) Wavelength selection Excitation 332/9 Monochromator Emission 1 488/15. com 10. 8. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 40266 Email: drugdiscoverytech@lifetech. AquaMax® 2000. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SpectraMax Mini: 17. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. Configure your microplate reader. Cyclic AMP titration curves were evaluated using SpectraMax Paradigm and SpectraMax M5e instruments. #M5e) Methods Microplate reader compatibility test. Microplate Reader, For Lab. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. A suspicious death, an upscale spiritual retreat, and a quartet of suspects with a motive for murder. 5. 91. Choose cables that meet the requirement described in this appendix. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 2 x 10-14. 8. Protein melting curves were recorded using CFX96 thermocycler (Bio-Rad). The SpectraMax M5, M5e, and. HTRF cAMP calibration curve HTRF cAMP calibration curve measured on the SpectraMax i3 (red circles), SpectraMax. Open the SoftwarePro® Data Acquisition & Analysis software,. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. 1. SpectraMax M5e: 118. Italic and boldface type are used for emphasis. 1. The guide also covers the software features, the specifications, and the accessories of the systems. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. FP assay do not perform well with protein:protein interactions. M3-M5e_manual. This document is provided to customers who have purchased Molecular Devices, Inc. 1. spr SpectraMax iD5 SpectraTest ABS2 v1. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of. Oxygen consumption is one of the most informative and direct measures of mitochondrial function. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. book Page 1 Wednesday, July 7, 2010 2:09 PM. reader (TUNE) SpectraMax M5e. 8. ISM Equipment Classification (Group 1, Class A) This equipment is. Testing we performed: The Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader passed all tests and was found to be fully functional: Power: PASSED Initialization: PASSED Display/Buttons: PASSEDThe Challenge. The analysis capabilities of the software provide automated quantitation in an easy-to-read, user-customizable report format. Jeffrey Manber, Managing Director of NanoRacks said, "Having the SpectraMax M5e Reader on-orbit will allow researchers to undertake research with real-time results. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. book Page 55 Wednesday, July 7, 2010 2:09 PM. Choose cables that meet the requirement described in this appendix. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Two cynomolgus monkeys (1 male and 1 female) weighing 2–4 kg and 3–5 years old were purchased from Suzhou. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. SpectraMax M5e: 118. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. elegans, and Alcanivorax borkumensis. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader with One Year Warranty. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. SoftMax Pro 5 GxP Software provides all the data collection and analysis tools of the standard edition, along withMolecular Devices. Setup Guide On The Molecular Devices SpectraMax®SpectraMax L: 36. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Lift up the lid over the chamber to insert or remove a cuvette. 3. Commenting on the launch, Christopher Silva, Director of Life Sciences Marketing at Molecular Devices, stated, "The HTRF(R) assay platform has given researchers an alternative set of kinase and GPCR assays, in. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. KeySpan adapter for SoftMax Pro and SpectraMax readers. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. You must scan the fluorochrome of interest in the SpectraMax M5 or M5e reader to determine the optimal excitation and emission wavelengths for your application. Optimal instrument settings are the same for all three readers. 1. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. SpectraMax M5e: 118. Endpoint, kinetic, spectrum. Devices SpectraMax M5e (Ex/Em 380/650nm) • SoftMaxPro software allows easy data analysis Real-time data analysis SoftMaxPro software allows easy viewing of oxygen consumption profiles in all wells (Fig. 00 USD. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. . 8 x 10-15. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. SpectraMax® iD5. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. 1. Once the M5e Reader is operational, space-based researchers will be able to conduct analysis on-orbit. In particular, these parameters should be entered as defined in the table below. D 1 1. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Path Check Pathlength Measurement We would like to show you a description here but the site won’t allow us. Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The SpectraMax M5e will debut today at the American Association for Cancer Research Annual Meeting in Washington, D. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seFigure 2: Expression of mCherry with (improved BBa_K1141000) and without (BBa_K1141000) the translation enhancing 5’ untranslated region was measured (Ex: 587 nm, Em: 610 nm) over a period of 6 hours after induction with 0. SpectraMax® ABS. 1. 5. The x and y calibrations are the same 60 µm per pixel 2. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. SpectraMax M Series Multi-Mode Microplate Readers | MolecularSpectraMax L: 36. The detector is a CCD array with 512 pixels in x and 512 pixels in y. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter. SpectraMax Glo and DuoLuc. The mirror set. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 2 x 10-14. Real-time fluorescence signals were recorded using CFX96 thermocycler (Bio-Rad). 2222 Fifth Street. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. SpectraMax Paradigm. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromM3-M5e_manual. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode Multi-mode Microplate Readers. 1 & 7. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well NanoRacks, LLC is installing a new, reconfigured Molecular Devices’ SpectraMax M5e multi-mode microplate reader on the International Space Station to perform experiments in microgravity. 8 x 10-15. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se在2011年5月,凭借其耐久性,Molecular Devices SpectraMax M5e成为了第一台进入太空的微孔板读板机。NanoRacks将它加入国际空间站中的商业硬件实验平台,首次为科学家提供了在微重力环境下进行微孔板检测的机会。Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. SUNNYVALE, CALIF. After the firefly luciferase was measured, Dual-Glo Stop & Glo substrate solution (25 µL/well) was added offline to each well to quench the firefly luciferase reaction and provide substrate for the Renilla luciferase reaction. SpectraMax M5e: 118. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 1(a). Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Cells were allowed to attach and grow overnight at. 2600A. 0 out of 5 $ 24,695. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 2 x 10-14. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Each tube of fluorophore also contained ROX. 1 or greater versions. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Add to Cart. SpectraMax M5. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Italic and boldface type are used for emphasis. Paradigm: W = 21. 5 mP standard deviation at 1 nMF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 00. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 0. 4, Z’ = 0. 8 x 10-15. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. SpectraMax M5e Multi-Mode Microplate Reader. Moving a SpectraMax Multi-Mode Microplate Reader. Using. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range. At the low light level (approaching the Lower. The drawing shows a standard microplate of 15 mm height covered with a lid. Add to cart. Proceed with the following steps using optimized settings. Call 516-939-0888 to check current. the SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Page 75. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 2 x 10-14. It does not work as expected. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. reader. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. measured using a microplate reader (SpectraMax M5e, Molecular Devices, San Jose, CA, USA) with excitation and emission at 494 and 520 nm, respectively, and calibrated against a standard curve for FITC. Bookings are free, but you will need to use iLab to book. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Our absorbance plate readers feature our PathCheck Sensor. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available) ATG for AKTA Explorer, Purifier and FPLC PMs, Repairs and Service Contracts. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Z1. 3. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. 313 reference gain check fail. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Molecular Devices SpectraMax M5e Microplate Reader. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. For more sample throughput, the system canSetup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. spr * SpectraMax i3(x) SpectraTest ABS2 v1. 1. HTRF is compatible with a large range of available microplate readers. Microplate Reader Shelf. Features. G-protein coupled receptors signal through two major pathways, regulation of cAMP and increase in intracellular calcium levels mediated by IP3. Toxicokinetics studies on cynomolgus monkey. 1. View the Molecular Devices website. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Molecular Devices. 5 mP standard deviation at 1 nM. 1. The corrected absorbance is within 5% of the value. 8 x 10-15. book Page 1 Wednesday, July 7, 2010 2:09 PM. M3-M5e_manual. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Like the SpectraMax M5 system, the SpectraMax M5e has primary applications for fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization,. 1. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The chamber contains springs that position the cuvette in the proper alignment for a read. 1. 6 x 10-15. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. SpectraMax L: 36. The optical systemsProducts : SpectraMax M5/M5e reader Can BRET be read on the SpectraMax M5? BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. book Page 1 Wednesday, July 7, 2010 2:09 PM The Molecular Devices SpectraMax M5e Microplate Reader enables five modes of detection. 5. 6 M HCl for 24 h. British Columbia, Selkirk Mountains. The schematic of the UED setup is shown as Fig. Figure 1. SpectraMax i3 reader SpectraMax Paradigm reader SpectraMax M5e reader cAMP EC 50 (nM) 19. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. e. Relative fluorescence units (RFU) were converted to units of NADPH concentration (μmol/L) using a standard curve of NADPH. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです! NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。SpectraMax ® ® M5e readers must be appropriately configured for HTRF ® readout by setting up the measurement conditions in the SoftMax Pro software. cAMP HiRange 0. 312 gain calibration failed. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 3. 485. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 5 Z’ factor 0. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Meso Scale Discovery 1250 Sector Imager 2400 $ 2,500. Conclusions. Labsystems Fluoroskan Ascent FL Microplate Fluorometer. This is a used Molecular Devices SpectraMax Gemini XS Microplate Fluorometer. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. Microplate Readers include the M3, M4,. Page 75. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. We also buy, refurbish, and resell used lab equipment. 1. A neurosurgeon from Mercer Island and a noted mountaineer from Spokane, Washington, were killed on February 4, 1986, when. 306 plate air cal fail,low light. for Cisbio Bioassays HTRF® technology. 41 nM were calculated using SoftMax Pro Software. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. SpectraMax® L (software version 5. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAn example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax® Aquamax. 6 x 10-15. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. Rating: 5. Open the SoftMax Pro Software and select TR-FRET for the Read Mode and Endpoint for the Read Type. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 1. Listing SKU: DIS-58825-molecular-devices-spectramax-m5e-microplate-readerIn May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader in space. SpectraMax M2. , 19 May 2011. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Fluorescent Polarization: Solid black plates, as above. The optical systemsSUNNYVALE, Calif. The user manual is an indispensable documentation for of any product. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. The donor and acceptor can be used to label a variety of biomolecules, for applications. The following document is intended to demonstrate setup of this instrument. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Description 1. book Page 21 Wednesday, July 7, 2010 2:09 PM. With optimized reagents, option to operate the readers. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. 315 can't find zero order. com A. Jul 13, 2023 10am PT Date Format. 6 x 10-15. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). A fluorescence microscope with high-throughput screening capabilities (Axio Observer. Introduction. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Fluorescence polarization was measured using SpectraMax M5e plate reader (Molecular Devices): 485 nm excitation (9 nm bandwidth), 525 nm emission (15 nm bandwidth). Bioz Stars score: 86/100, based on 1 PubMed citations. 2. 4. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. The SpectraMax M2 and M2e are versatile and reliable. SpectraMax ABS/ABS Plus SpectraMax i3/i3x MiniMax SpectraMax iD3/iD5 Spectramax 190 EXT Spectramax 340PC/384 Spectramax Plus 384 Spectramax L Spectramax M2 Spectramax M2e Spectramax M3 Spectramax M4 Spectramax M5 Spectramax M5e Spectramax Mini Versamax EXT Vmax Microplate Washers;. The BRD4 bromodomain 1 TR-FRET assay was performed as described in the assay kit manual. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. 5 mM IPTG using a SpectraMax M5E. The following document is. The SpectraMax iD5 and SpectraMax i3x readers use SoftMax® Pro Data Acquisition and Analysis Software. Cell monolayers were decalcified with 0. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. ZERO BIAS - scores, article reviews, protocol conditions and moreThe NanoRacks Plate Reader-2 is a reconfigured SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, and has been configured to operate comfortably in the microgravity environment of the space station. Categories:. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart Features We would like to show you a description here but the site won’t allow us. Delivers single mode reader performance in one multimode reader package. Download PDF. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. 4. the SpectraMax M5e, one of Molecular Devices most reliable and feature-rich microplate readers, to operate comfortably in the zero-gravity environment of the space station. The optical systemsThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). spr SpectraMax iD3 SpectraTest ABS2 v1. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. 4. Supplier: Molecular Devices. With optimized reagents, option to operate the. SpectraMax ABS Plus SpectraTest ABS2 v1. 5, Z’ = 0. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9The Molecular Devices SpectraMax M5e Microplate Reader enables five modes of detection. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 8 x 10-15. 1. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Ex wavelength (nm/bandwidth) 485/20. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Path Check Pathlength Measurement The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The corrected absorbance is within 5% of the. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. 01 1 100 10000 1000 2000 3000 SpectraMax Paradigm SpectraMax M5e [cAMP] nM delta F % EC 50 Values Paradigm = 18nM M5e = 19nM Variation of Integration Time-12 -10 -8 -6 -4 5000 10000 15000 20000 25000 50 s. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. When a feature or capability applies to only certain readers, this exception is noted. Table of Contents. ® reader to the computer. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Note: In this user guide, SpectraMax ® refers to several SpectraMax . reader (FI-FLRH) SpectraMax Paradigm. M5/M5e Multi-Mode Microplate Reader SpectraMax. Choose from a three- (M3), four- (M4), orHome; Lighting; Work lights; User manual. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromMolecular Devices SpectraMax™ M5e Microplate Reader w/ Warranty (Svc Contract Available) $34,000. For example, microplates containing different samp les can be read using the same orSpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. Venue Address. Reads various kinds of 96-wells plates, and is equipped with shaking function. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ.